Improvement of callogenesis and somatic embryogenesis by selecting optimal hormonal balance in Sarnav and Desiree potato varieties

Abstract Callus formation and somatic embryogenesis in potato varieties are effective ways to obtain an entire plant from a single cell. The implementation of is widely used improving materials creating new biotechnological varieties. In our research, the optimum level 1-naphthaleneacetic acid (NAA) 6-benzyl aminopurine (BAP) was determined callus tissue local Sarnav variety, Desiree variety taken as control. Leaf stem explants were processes both We tried various growth regulators for different ratios. 1 mg/L NAA 1.5 BAP established best option resulting 92 100% development internodes varieties, respectively. 0.1 gibberellic (GA 3 ) resulted almost 80% found most option. These results showed that regenerated plants can be obtained by embryogenesis. This method applied genetic transformation studies.